|
|
|
|
|
| Gatesoupe, Joel; Coves, Denis; Ortega, Aurelio; Papandroulakis, Nikos; Vadstein, Olav; De La Gandara, Fernando. |
| During the first 2 years of larval rearing trials with Atlantic bluefin tuna, survival was a challenging issue. As bacterial colonization of the gut has been shown to play a key role for other species, we studied the profiles of the microbiota associated with individual larvae at different stages in three distant hatcheries. The Bacterial Community Profile (BCP) was quantified based on PCR-DGGE analyses of partial amplicons from 16S rDNA. Considerable individual variability in BCP was observed before onset of feeding, and the BCP did not show regular fluctuation during ontogenesis. Microalgae were added to the rearing tanks in two of the three hatcheries, but it was not possible to distinguish the effect of location from the effect of algal addition on... |
| Tipo: Text |
Palavras-chave: Larval rearing; Bacterial diversity; DGGE; Thunnus thynnus. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00075/18648/16197.pdf |
| |
|
|
| Ghysen, Alain; Schuster, Kevin; Coves, Denis; De La Gandara, Fernando; Papandroulakis, Nikos; Ortega, Aurelio. |
| The lateral line system of amphibians and fish comprises a large number of individual mechanosensory organs, the neuromasts, and their sensory neurons. The pattern of neuromasts varies markedly between species, yet the embryonic pattern is highly conserved from the relatively basal zebrafish, Danio rerio, to more derived species. Here we examine in more detail the development of the posterior lateral line (PLL) in embryos and early larvae of one of the most derived fish species, the blue-fin tuna Thunnus thynnus, and of its close relative, the Atlantic bonito Sarda sarda. WE! show that the basic features of embryonic PLL development, including the migratory properties of the PLL primordium, the patterning of neuromasts and their innervation, are largely... |
| Tipo: Text |
Palavras-chave: Neuromast; Hair cell; Planar polarity; Cupula; Migrating primordium. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00019/13032/10162.pdf |
| |
|
|
| Mazurais, David; Coves, Denis; Papandroulakis, Nikos; Ortega, Aurelio; Desbruyeres, Elisabeth; Huelvan, Christine; Le Gall, Marie-madeleine; De La Gandara, Fernando; Cahu, Chantal. |
| The aim of this study was to determine whether mortality observed during the larval development of reared bluefin tuna (Thunnus thynnus) could be related to improper expression profiles of key genes involved in digestive or antioxidant response capabilities. Tuna larvae were sampled at hatching, 2, 5, 10, 15 and 20 dph (days post hatching) for the relative quantification of transcripts encoded by genes involved in digestive [trypsinogen 1 (TRYP1), alpha-amylase (AMY), aminopeptidase N (ANPEP)] and antioxidant [catalase (CAT)] functions. The levels of expression of ANPEP related to the development and maturation of intestinal function increased from 5 to 20 dph. Furthermore, AMY and TRYP1 genes, which are pancreatic enzymes implicated in carbohydrate and... |
| Tipo: Text |
Palavras-chave: Tuna; Gene; Expression; Development; Digestion; Antioxidant. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00171/28264/26521.pdf |
| |
|
|
| De La Gandara, Fernando; Mylonas, Constantinos C.; Coves, Denis; Ortega, Aurelio; Bridges, Christopher R.; Belmonte Rios, Antonio; Vassallo-agius, Robert; Papandroulakis, Nikos; Rosenfeld, Hanna; Tandler, A; Medina, Antonio; Demetrio, A; Corriero, Aldo; Fauvel, Christian; Falcon, Jack; Sveinsvoll, K; Ghysen, Alain; Deguara, S; Gordin, Hillel. |
| Tipo: Text |
|
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00029/14015/11205.pdf |
| |
|
| |
|
|
| Fauvel, Christian; Suquet, Marc; Severe, Armelle; Mylonas, Constantinos C.; Papandroulakis, Nikos. |
| The potential of wreckfish (Polyprion americanus) to breed in captivity was examined. Ovarian activity varied seasonally, with vitellogenesis beginning in November and ending in May. Slow-release implant with gonadotropin-releasing hormone agonist (GnRHa) prevented atresia, and both GnRHa injections or implants at the end of vitellogenesis triggered ovulation after 7 days. Spontaneous spawning was obtained only in one female, and the few eggs produced were of poor viability. Manual stripping and artificial fertilization produced viable gametes and embryos. Therefore, present captivity conditions inhibited final oocyte maturation (FOM) and spawning, but not vitellogenesis and spermatogenesis. GnRHa treatments stimulate FOM and ovulation of viable eggs. |
| Tipo: Text |
Palavras-chave: Aquaculture; Polyprion americanus; Wreckfish; GnRHa; Gametogenesis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6554.pdf |
| |
|
|
| Pérez, Evaristo; Linares, Fátima; Rodríguez Villanueva, José; Vilar, Antonio; Mylonas, Constantinos; Fakriadis, Ioannis; Papadaki, Maria; Papandroulakis, Nikos; Papadakis, Ioannis; Robles, Rocío; Fauvel, Christian; Roo, Javier; Peleteiro, José; Lluch, Nuria; Pazos, Gema; Méndez, Belén; Sigelaki, Irini; Gómez, Castora; Pérez, Montse; Álvarez-blázquez, Blanca. |
| Four different wreckfish (Polyprion americanus) broodstock batches were maintained in research facilities under different photo and thermo-period conditions, one in Greece, the Helenic Center for Marine Research (HCMR, n = 3) and three in Spain: Instituto Español de Oceanografía (IEO, n = 13) in Vigo, Aquarium Finisterrae (MC2, n = 21) in A Coruña and Consellería do Mar (CMRM, n = 11). The CMRM includes two centers that work together: Instituto Galego de Formación en Acuicultura (IGAFA) and Centro de Investigacións Mariñas (CIMA), both in Pontevedra. During the five years of the project DIVERSIFY (Exploring the biological and socio-economic potential of new-emerging candidate fish species for the expansion of the European aquaculture industry, 2013–2018)... |
| Tipo: Text |
Palavras-chave: Wreckfish; Broodstock; Larval husbandry; Nutrition; Natural spawns; Live prey; Lipid profile; Ontogeny. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00660/77192/78615.pdf |
| |
|
|
|
